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can you tell me if i need to separate my male from my female as she is pregnant
 

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You should not keep male and female rats together... Unless the female is spayed( which I doubt she is since she is pregnant) or if the male is neutered.
So please separate the male from the female :)
 

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In general, yes. The female may get more aggressive and if and when she gives birth he can get her pregnant again immediately, which is very unhealthy for her and the babies.

I would suggest keeping them separate and keeping a male baby and a female baby to keep them company and not putting them back together.

The male babies will have to be separated from the the females (and their mother) at 5 weeks.
 

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I am just wondering by the sounds oof it you were trying to breed the two considering that you had them in the same cage are they pet store rats ?
 
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